Absorbance was measured with a microplate reader (VERSAmax Molecular Devices Corporation, Sunnyvale, CA, USA) at 570 nm to assess cell viability. The optical density of each well at 490 nm was then measured using a microplate spectrophotometer system (VersaMax; Molecular Devices, Sunnyvale, CA, USA). Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Full size table. The absorbance was then determined by an enzyme-linked immunosorbent assay (ELISA) reader (VERSAmax; Molecular Devices, Sunnyvale, CA) at a wavelength of 405 nm. 4. Select up to two wavelengths. Versamax Microplate Reader dibangun di sekitar monokromator kisi. The average survival rates for cells exposed to dry. After freeze-drying, the. Biosystems CytoFluor 4000 Multi Well Plate Reader $ 1,995. Sale Sold out. . Statistical analysis Graphs and statistics were prepared in GraphPad Prism, version 7. Four wash/rinse bottles are included and configurable for both 96- and 384. Select Update Driver. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). Battery Backup Uninterruptible Power Supply (UPS) And Power Conditioner For MDS Sciex Molecular Devices VERSAmax Microplate Reader Quote Request. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers begining with. Indocyanine Green Depuration Assay. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. This. This manual does not represent a commitment on the part of Molecular Devices Corporation. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 2 0112-0126B ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevicesequipment,software, SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Model : VersaMax . VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 11 0112-0126B Theguidelinesforcuvetteuseintheinstrumentarethesameasthosethatapplytoany high-qualityspectrophotometer. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. TECHNICAL NOTE. Spectrofluorometer (e. $1,195. 00 USD Tecan Infinite F200 Reader Fluorescence Reader w WARRANTY $2,750. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 2 0112-0126B ThisdocumentisprovidedtocustomerswhohavepurchasedMolecularDevicesequipment,software,SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 6/10 #0120-1330d. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The absorbance at wavelength 450 nm was detected using a micro-plate reader (VersaMax, Molecular Devices, USA). +06-6399-8211 Fax +06-6399-8212 ©2002 Molecular Devices Corporation. After three times washing, the reaction was colored by substrate 3,3’,5,5’-Tetramethylbenzidine (TMB) (T0440, Sigma), and stopped by 2 N HCl. Multichannel optics. StakMax Microplate Handling Stacker. The percentages of inhibition of cell viability were calculated as the ratio between the absorbance of the compound-treated cells and cells with only the vehicle. And the VERSAmax reader is robotics-compatible. The SpectraTest® ABS1 Absorbance. The Molecular Devices SpectraMax XPS has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. 滤光片型光吸收微孔板读板机（酶标仪），简单易用，性价比高，检测结果精确. Taking into account the price differences between each seller and each country. Molecular Devices Spectramax Plate Reader. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pm SpectraMax and VersaMax . It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 0%, 0-2. SpectraMax®, VersaMax, VMax. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer. Add to Cart. Molecular Devices versamax Operator's Manual (47 pages) Tunable Microplate Reader. Never order or replace filters to optimize an assay again! Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. Tristar 3 Multimode Microplate Reader. Molecular Devices VersaMax Tunable Temperature range between 4°C above ambient to 45°C Digital controller and display Sold as-is The Molecular Devices VersaMax microplate spectrophotometer provides rapid and sensitive measurements for a variety of analytes across a wide range of concentrations. 0 OD Kinetic Read Time: 9 Second Minimum Interval Temperature Uniformity ±0. This technical note is designed to provide information . Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The water extract afforded the greatest yield, and total phenolic and tannin. VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 11 0112-0126B Theguidelinesforcuvetteuseintheinstrumentarethesameasthosethatapplytoany high. SoftMax® Pro Software Validation Package provides the most comprehensive documentation and tools available to validate GxP administrator features, software. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. StakMax Microplate Handling System. The final mixtures were allowed to react at 25 °C in the dark for 5 min. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Molecular Devices Versamax Absorbance Microplate Reader BN 02970. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochromator-based systems. 3 cells (6x105) were ocExtracts were placed in a 96-well plate in triplets and spectrophotometric readings were taken at 555 nm on a microplate reader (Versamax, Molecular Devices LLC, USA). to 11. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. A. Glossary of Terms. Select Start>Control panel>Device Manager. The colorimetric reaction was read using an automated ELISA microplate reader (Versamax, Molecular Devices, USA) at 492 nm. The culture supernatant of cells (25 μl) was mixed with 225 μl of DMMB dye solution and the absorbance of solution mixture was measured at 525 nm with a spectorophotometer (VERSAmax; Molecular Devices Japan KK, Tokyo, Japan). Culture medium was removed, and lysis buffer (10 ml) was pipetted into each well. DMEM medium supplemented with the same volume of kit reagent on an empty well was used as a blank. That's why our team is at your disposal the majority of time. 00 Sale price $ 0. It features a dual-monochromator system, a large dynamic range, and a high-throughput capability. It features a modular design that allows users to add or change detection cartridges according to their needs. 00 Read more Molecular Devices Tunable Microplate Reader VersaMaxMicroplate reader with 450 nm filter, such as VersaMax (Molecular Devices, Sunnyvale, CA). VERSA MAX-LUNABLE, MOLECULAR DEVICES, MICROPLATE R. 5 million deaths worldwide have been caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (), along with high levels of unemployment and far-reaching supply chain issues in the world’s economy. The index was evaluated at 48 and 72 hours normalized to that at 24 hours. printed in u. ). 0 out of 5 $ 23,995 $ 22,495. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Absorbance plate reader (e. Cell. 17. The conversion of L-DOPA to dopachrome was allowed to proceed for 40 min at 30 °C in a microplate reader (Versamax; Molecular Devices). Results are presented as the geometric mean and are derived from at least three experiments. The in vitro differentiation of BMDMs. (VersaMax Molecular Devices, Sunnyvale, CA). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes. A. developing diagnostic and research tools using. TheMolecular Devices VersaMax Microplate Reader (ZB64) . The absorbance was measured at 490 nm using a VersaMax ELISA reader (Molecular Devices, Sunnyvale, California, United States). The absorbance was assumed to be 100% for the control group. 8). After 4 h of incubation at 37 °C, culture medium was discarded and dimethyl sulfoxide (150 μL/well; Sigma) was inoculated to dissolve the precipitated formazan produced in viable cells. 2 and 648. The reaction was started by adding 10 µL of the enzyme solution (properly diluted) to 190 µL of 15 mM ONPG in 0. BMG Labtech - THERMOstar. The samples were incubated with FBS, and the optical density of particles was determined with a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA) by measuring the absorbance at 560 nm for 2 h. ¨Molecular Devices is a life-science technology innovator Our integrated hardware and software solutions enable automated workflows—from start to finish—for any research application. Molecular Devices FMax Thermo Fluoroskan Ascent Fluorescence Microplate Reader W WARRANTY $3,450. 0 OD. S. Access all the resources, tools and information you need to stay productive. We use. The rate of cell viability was calculated using the following formula:The conversion of NADP+ to NADPH was measured as absorbance (340 nm) on a microplate reader (VersaMax, Molecular Devices Co. SoftMax® Pro, the industry-leading microplate reader software, is included at no additional charge. Cell viability was quantified by microplate reader (VersaMax, Molecular Devices, San Jose, CA, USA) at 570 nm. Molecular Devices Corporation SpectraTest ABS1 Validation Plate Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1. Monochromator, tunable in 1-nm increments. Conventions Used in this Manual. All samples were measured in triplicate. The limit of detection for each assay was 156. Cell cycle investigation. These values were transformed to logarithmic scale to create a log-log curve. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. However, whether these cells exhibit the serotonergic neuronal phenotype observed in mature hippocampal neurons. Our absorbance plate readers feature our PathCheck Sensor technology and industry-leading SoftMax® Pro Data Acquisition and Analysis Software. 00. The VersaMax Absorbance Microplate Reader allows for selection of. Find regional support. HL-60 cells were plated at a density of 3×105 cells/well on 6-well plates. In addition, the dissected tissues (heart, liver, spleen, lung and kidneys) were fixed in 4% paraformaldehyde, embedded in paraffin, and sliced into 4-μm thick sections. Using a Spectradrop Micro-Volume Microplate (VersaMax, Molecular Devices, CA, USA), RNA was quantified, and 1 μg of total RNA was treated with DNase I (Thermo Scientific, Waltham, MA, USA) before. Finally, 0. 2. 8), 40 μL of tyrosinase, and 40 μL of L-DOPA was put in each well. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Antioxidant enzyme activityProtein concentration was determined from diluted crude venom and nanoparticle samples (1 mg/mL) in deionized water by BCA™ Protein Assay using a UV/V spectrophotometer (VersaMax, Molecular Devices, CA, USA) at a wavelength of 562 nm. 2 mM PLP and 5. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Molecular aspects of color variation in luciferase emission have been the subject of numerous studies: various mechanisms have been proposed [6] and multicolor reporter proteins have been created and applied in cell-based. ¨We would like to show you a description here but the site won’t allow us. It also integrates with the powerful SoftMax Pro Software for. Cell-free AB broth (22) of an overnight culture of the acylhomoserine lactone-defective V. With a wavelength. Quantitative real-time polymerase chain reaction For quantitative real-time polymerase chain reaction (qRT-PCR) analysis, total cellular RNA was extracted from ten zebrafish larvae. Study conductThe amounts of chlorophyll a and chlorophyll b were measured at 664. Fabrication of Microfiber-Based Strain SensorsWe would like to show you a description here but the site won’t allow us. 1700 +1 800. The signal produced was proportional to the amount of analyte present and interpolated from the calibration curve presented on each plate. Concentration was determined by BCA Protein Assay Kit (ref. Data were analyzed with GraphPad Prism. The cells were treated with 15, 25, 30, and 45 nm-particles in order to compare the cytotoxicities of different sized nanoparticles on the treated cells. The colour change of the MTS solution was measured at 490 nm by using a tunable microplate reader (VersaMax Molecular Devices, USA). Values are presented as the mean ?standard deviation (SD). This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. SpectraMax® i3 Multi-Mode Detection Platform. ¨ Molecular Devices is a life-science technology innovator Our integrated hardware and software solutions enable automated workflows—from start to finish—for any research application. GABase was a mixture of γ-aminobutyrate glutamate amino transferase. The cortisol-driven molecular programming of HPI axis development has focused only on GR signalling in fishes 12,13,14,15,16,17. Sale Sold out. Formerly known as MicroMax, Molecular Devices’ unique SpectraDrop™ Micro-Volume Microplate, offers the highest throughput solution for low volume measurement available onthe market today. 3, Molecular Devices). The activity was measured colorimetrically at 450 nm using a microplate reader (Versamax, Molecular Devices, Sunnyvale, CA, USA). 1 M sodium phosphate buffer (pH 6. The VersaMaxTM Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. ,, [28]. Study conductThe amounts of chlorophyll a and chlorophyll b were measured at 664. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. 1. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. ®SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. This. The robot-compatible VersaMax Reader is cost-effective—users never have to buy filters. . The value for non-specific absorption at 600 nm was subtracted. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. For more information, please visit us at 160 µL of sample solutions/positive control (ascorbic acid) were mixed with 40 µL of DPPH solution and incubated at room temperature for 30 min. Molecular Devices VersaMax Microplate Reader. 00 USD. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Acetylcholinesterase activity was calculated as the rate of change of absorbance per minute during the linear portion of the reaction and normalized by protein concentration as determined by a Coomassie. Accession codes. MOLECULAR DEVICES VersaMax is a customizable lab equipment capable of performing multiple types of research-grade tests and experiments at once, with enhanced safety protocols and adjustable column and sample-loading system for efficient sample preparation and increased throughput. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. +81-6-6399-8211 fax +81-6-6399-8212 Nihon Molecular Devices Tokyo Tel. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. All parts of the SpectraTest ABS1 Validation Plate are warranted for a period of ninety (90) days from the original date of delivery. Reboot the computer. 68 $. pdf file (03 Dec 2022) Manufacturer: Molecular Devices; Category of Device: Laboratory Equipment, Measuring Instruments; Document: versamax Operator's Manual, File Type: PDF;Search for used versamax. Nude mice were obtained from Orient Bio Inc. together with SpectraMax, VersaMax, VMax, and EMax microplate readers from Molecular Devices, offer a quality assurance screening solution for the detection of melamine contamination in such food products as milk, infant formula, pet food, and confectionery. One microgram of RNA was. After addition of stop solution, the optical density of samples were read at wavelengths of 450 and 570 nm (Versamax, Molecular Devices Co. Windows 10 – 2004/20H2/21H1/21H2. The blue formazan concentration formed in viable cells was measured at 570 nm using spectrophotometer (Versamax, Molecular Devices, São Paulo, Brazil). To purchase a newer software version, request a quote. Animals and preparation of tumor model. 3 Methods 1. SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. The emodin thermoreversible PG was. Absorbance was read at 412 nm every minute for 10 min using a VersaMax (Molecular Devices, San Jose, CA) spectrophotometer. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. To measure the luciferase activity biochemically, cells wereThe activity was measured colorimetrically at 450 nm using a microplate reader (Versamax, Molecular Devices, Sunnyvale, CA, USA). The plate was read at 570 nm after 5 min using Versamax (molecular devices, United States). Cell migration assay. Statistical Analysis. Manufacturer: Molecular Devices The Molecular Devices VersaMax Tunable Microplate Reader for 96-well format uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. SpectraMax Plus384 SpectraMax 190 SpectraMax 340PC384 VersaMax Microplate Spectrophotometer Operator s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The mixture was incubate at 37 °C for 30 min, and then the reaction was stopped by the addition of 0. from Molecular Devices Corporation. DNA content in the supernatant (100 μl) was measured using a Quant-iT PicoGreen assay kit (Invitrogen) as per the manufacturer's instructions. 9000-0756. ¨ We would like to show you a description here but the site won’t allow us. We would like to show you a description here but the site won’t allow us. Our mission: find your user guide or user's manual as quickly as possible. Cell survival rate of each sample was calculated as a percentage relative to cells incubated in D-MEM. 1% (w/v) naphtylethylenediamine-HCl], incubated at room temperature for 10 min, and then the absorbance at 540 nm was measured in a microplate reader (VersaMax™, Molecular. They were then pre-treated with indicated concentration of drugs for 1 h prior to being treated or not with 5-HT or serum. The optical densities (ODs) of the reactants were measured at 450 nm using a microplate spectrophotometer (Versamax, Molecular Devices, CA, USA). The. SoftMax® Pro, the industry-leading microplate reader software, is included at no. All the experiments were conducted at. All analyses were performed three times, and the mean values were used for further calculations. 9. Myeloperoxidase AssayThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Invasion assayCells were seeded into the top chamber of a 96-well matrigel-coated plate with 8-m-pore polyethylene terephthalate membrane inserts (Corning). Home - University of Victoriaimported directly into Excel (Microsoft, Redmond, WA) from the ELISA microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). S. Wavelength Selection. The system provides automation for up to 50For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Used. Molecular Devices VersaMax Microplate Reader. No accessories are needed and SoftMax® Pro Software. US HEADQUARTERS. For determination of the optimal temperature, EfmTDC was incubated in 100 mM sodium acetate buffer (pH 5. However, whether these cells exhibit the serotonergic neuronal phenotype observed in mature hippocampal neurons. The absorbance was detected at 570 nm with a Microplate Reader (VersaMax, Molecular Devices, Sunnyvale, CA). Cells were treated with 150 μl of dimethylsulfoxide (DMSO) and absorbance was quantified at 540 nm using the microplate spectrophotometer system (VersaMax, Molecular Devices, Sunnyvale, CA, USA). Finally, the absorbance was determined at 490 nm on a microplate reader (VersaMax, Molecular Devices, Sunnyvale, CA, USA). Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). Download PDF versions of the SpectraMax Microplate Reader User Guides. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The emodin thermoreversible PG was. For more sample throughput, the system can be easily integrated into a full roboticThe VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. To evaluate the reliability of the assay, SkinEthic TM RHE (1% Triton X-100) was used. (Molecular Research Center, USA) according to the manufacturer’s. The absorbance was read with a multiwall spectrophotometer (VERSAmax, Molecular Devices, Sunnyvale, CA, USA) at 450 nm. Concluding Remarks. Cell proliferation assay MDA-MB-231 cells were seeded at a density. 747. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Manufacturer: Molecular Devices Model: VERSA MAX Turnable Microplate reader Further Information Descriptions, data, dimensions and quantities are based on the subjective beliefs of the auctioneer and are created to the best of our knowledge and belief. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Used. The BMDM cells were plated into a 6-well culture plate at a density of 2. Furnace Manuals. We would like to show you a description here but the site won’t allow us. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Molecular Devices Corporation. Cell viability was represented by percentage values compared to a. JEG-3 cells were transfected with myc-tagged NDRG1 wild type or deletion mutants at the N- or C- terminal as depicted in Figure 5A. We would like to show you a description here but the site won’t allow us. Flow Cytometry AnalysisAt the end of the exposure, the MTS solution (20 µL/well) was added, and the cells were incubated for 3 h at 37°C. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. Biosystems CytoFluor 4000 Multi Well Plate Reader $ 1,995. Absorbance was read at 412 nm every minute for 10 min using a VersaMax (Molecular Devices, San Jose, CA) spectrophotometer. 00 Regular price. Molecular Devices Beijing Tel. BMG Labtech - THERMOstar. g. Electrolyte leakage analysis was conducted using 8-day-old rice seedlings before and after cold stress treatments in different time points (0, 6, and 11 days) at 4°C. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. MOLECULAR DEVICES VersaMax Reader: Model #: VersaMax: Serial #: N/A: Year Built: N/A: Location: California: Price: $3,800. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. We would like to show you a description here but the site won’t allow us. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. This version is compatible with the following instruments: SpectraMax® i3x Multi-Mode Detection Platform. Finally, the optical density of the wells was determined at an excitation wavelength of 450 nm and a reference wavelength of 540 nm on a VersaMax (Molecular Devices) microplate reader. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. 8. In the Apps and Features window, locate and uninstall SoftMax Pro. the plate reader (model VERSAmax, Molecular Devices, USA) at 515 nm (A515). Windows Home Editions are not supported. Unit price / per . This manual is subject to change without notice. 2. , Sunnyvale, CA, USA). The objectives of this study were to determine phytochemical compositions, chemiluminescence antioxidant activities, and neuroprotective effects on PC12 cells for water, methanol, and 95% ethanol extracts of the air-dried fruit of <i>Terminalia chebula</i> Retzius. , Sunnyvale, CA). Bovine serum albumin was used as a reference. The Molecular Devices VersaMax ELISA Microplate Reader enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing. Global research efforts are focused on understanding the SARS-CoV-2 virus in order to develop potential therapies for COVID-19 including: vaccines. Coat plate with 100 μL each of appropriately diluted standards (Table 1),. The wells were visualized using o-phenylenediamine (OPD), and the absorbance was read with a multiwall spectrophotometer (VERSAmax, Molecular Devices, Sunnyvale, CA, USA) at 490 nm. The assay was performed in 96-well microtiter plates in duplicate, and the absorbance was read at 405 nm with 620 nm as the reference wavelength using a microplate reader (VersaMax, Molecular Devices, Boston, Massachusetts). All cell lines were cultivated for 24 h in 96-well cell culture plates with an initial concentration of 1×10 5 cells/mL. Youmustensurethatthemeniscusiscomfortablyabove thelightbeaminstandardcuvettesandthatthesamplechamberinamicrocuvetteis Microplate Reader Capability > UV - Vis Absorbance. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. , St. For pinocytosis, cells in wells were washed in PBS and 5 mg/ml of HPTS dye (in PBS) added and incubated with gentle rocking at 30 °C for 120 min. (Seongnam, Korea). To evaluate the reliability of the assay, SkinEthic TM RHE (1% Triton X-100) was used. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 7. 4 Microplate Reader Software w WARRANTY. The nuclear extracts were subsequently determined for the content of NF-κB with the NF-κB (p65) transcription factor assay kit. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. In brief, mature 3T3-L1 cells were washed with DPBS, fixed with 10% formalin for 1 h at room temperature, washed once with 60%. , VersaMax; Molecular Devices) Incubator for cell culture. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Molecular devices The VersaMax Absorbance Microplate Reader costs between $5,500 and $6,600. ces at one of the following telephone numbers: 408 747-1700 800 635-5577 U. The catalytic activity was calculated from the production rate of the hydrolyzed product, p-nitrophenol, by monitoring the absorption (405 nm) at room temperature. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. For the MTS assay, the CellTiter 96® AQ ueous One Solution Cell Proliferation Assay kit was used following the manufacturer's instruction. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. SoftMax® Pro 5 Software from Molecular Devices offers extensive data capture, analysis, and reduction capabilities for its absorbance, luminescence,. Proliferation was assessed by bromodeoxyuridine (BrdU) incorporation (Cell Signaling Technologies). 99 shipping. 2. Absorbance was determined at 490 nm on a microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). DMEM medium supplemented with the same volume of kit reagent on an empty well was used as a blank. A standard curve, provided by the manufacturer, was generated and the sample concentrations determined by 4-parameter logistic curve. The system provides automation for up to 50 For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. The signal was read on an ELISA plate reader (SpectraMax VersaMax, Molecular Devices, Sunnyvale, CA, USA). Molecular devices The VersaMax Absorbance Microplate Reader costs between $5,500 and $6,600. The plate was read at 570 nm using an ELISA reader (VERSAMax, Molecular Devices, VWR, Philadelphia, PA, USA). ) gap between the rear of the instrument and the wall. Briefly, at 3 h before each of the desired time points, 10 µl of the MTS reagent. Optical Densitiy (OD) values were processed with a data reduction software (SoftMax Pro, Molecular Devices) using a 4-parameter logistic fitting algorithm to the standard curve, allowing the determination of antibody. Related products. 0 nm full width half maximum. The catalytic activity was calculated from the production rate of the hydrolyzed product, p-nitrophenol, by monitoring the absorption (405 nm) at room temperature. VersaMax Absorbance Microplate Reader memungkinkan pemilihan hingga dua panjang gelombang sekaligus untuk deteksi absorbansi dalam. 4. SpectraMax Plus384, 190, 340PC384, and VersaMax User Guide Molecular Devices User Guide SpectraMax. ® Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. 00 Read more Molecular Devices Tunable Microplate Reader VersaMaxMicroplate reader with 450 nm filter, such as VersaMax (Molecular Devices, Sunnyvale, CA). Softmax Pro 소프트웨어가 포함된 PC 워크스테이션 포함. An aliquot of the samples dissolved in DMSO with 80 μL of phosphate buffer (pH 6. This manual is subject to change without notice. This document is provided to customers who have purchased Molecular Devices, Inc. To test if sodium arsenite and dimethylarsenic acid could stimulate apoptosis in 3 cells, oc cell cycle redistribution was studied using flow cytometry with propidium iodide staining. The preparation of cytoplasmic and nuclear proteins was performed by using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Invitrogen, USA) according to. Tunable, filterless flexibility The. Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial. After washing in PBS at least 4 times to remove all extracellular dye, samples were fixed using 4 % PFA/PBS for. 5°C @ 37 °C W. We would like to show you a description here but the site won’t allow us. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. With optimized reagents, option to operate the. 0200-6117. 00: Description. (VersaMax, Molecular Devices, Sunnyvale, CA, US). We would like to show you a description here but the site won’t allow us. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The absorbance was measured at 450 nm with 650 nm as a reference on a VersaMax (Molecular Devices, Sunnyvale, CA) and a standard curve was plotted using a 4-parametric mathematical fit model in. Statistical analysisThe absorbance was measured using a microplate reader (VERSAmax; Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 530–560 nm. Briefly, MTT (200 μl, 2 mg/ml) was added to the wells and incubated for 4 h at 37°C. Molecular Devices Corporation SpectraTest ABS1 Validation Plate Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. The number of cells that migrated to the bottom was determined by measuring the optical density at 590 nm, using a microplate reader (VersaMax; Molecular Devices). The sections were stained with hematoxylin and eosin (H&E) and. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Molecular devices Versa Max. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Oil red O staining. Select Update Driver. P. A. Regular price $ 0. SoftMax® Pro 7. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. After 30-min incubation, the absorbance was measured at 405 nm wavelength using a spectrophotometer device (VersaMax Molecular Devices, Sunnyvale, CA, USA). This technical note is designed to provide information .